In this review, we propose an overview of the different techniques used to produce microsized aqueous compartments confined by a single lipid bilayer that separates them from the surrounding aqueous environment.
Microfluidics and lab-on-a-chip technology have emerged as the most promising approaches to address these challenges. These innovative technologies hold the potential to unlock new insights into single cell properties and their roles within populations.
Droplet microfluidics is a powerful tool which consists of generating and manipulating micron-sized monodispersed droplets.