Automated Multiplexed Imaging Platform

What is multiplexed imaging?

Multiplexed imaging refers to a technique used in biological imaging to simultaneously visualize multiple targets or biomarkers within a sample. It allows researchers to obtain spatial and molecular information from a single sample, providing a more comprehensive understanding of complex biological systems. In traditional imaging techniques, such as immunofluorescence or immunohistochemistry, only a limited number of targets can be visualized at a time using specific fluorescent labels or antibodies. Multiplexed imaging expands the capability by enabling the detection of numerous targets in a single experiment. 

There are various approaches to achieve multiplexed imaging. One common method involves using different fluorophores or fluorescent dyes that emit distinct wavelengths of light. By selecting fluorophores with non-overlapping emission spectra, multiple targets can be labeled and detected simultaneously. This technique is often referred to as multiplex fluorescence imaging. 

Multiplexed imaging has significantly advanced our understanding of complex biological processes, such as cell signaling, tissue microenvironments, and disease pathology. By enabling the simultaneous detection of multiple targets within a sample, researchers can gain deeper insights into the spatial relationships and interactions between different biomolecules, cells, or tissues, leading to more comprehensive and detailed biological analyses. 

Description of the multiple fluid delivery platform 

The automated multiplexed imaging platform is the perfect compromise between manual pipetting and an all-in-one system dedicated to one specific application, integrating a microscope, a specific chip type, and a given set of solutions. Any protocol with multiple solution delivery can be automated, saving the scientist time and reducing variability between experiments compared to manual procedures. 

The platform, which integrates our ARIA automated sequential injection system, is straightforward and ready to use. No specific training is required, and no time is lost troubleshooting incidents or manually priming solutions before the experiment as with custom-built systems.