Immunostaining Pack

O-MULTILABEL-PCK

A ready-to-use package for sequential injection of multiple fluids for cell or tissue staining

The Immunostaining Pack provides stable and automated delivery of up to 10 different fluids to a single cell culture chamber.

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Main benefits

Easy
Simple connections
Designed for cell biology
Contamination-free, Sterile environment
Compact
Inject up to 10 different samples
Versatility
Possibility to heat or agitate reservoir

Why use sequential injection for immunostaining?

Immunostaining is a multi-step technique for detecting the presence or localization of specific proteins, molecules, or cells within a biological or tissue sample.

Various reagents such as fixating agents or permeabilization buffers, are sequentially added to the samplewith incubation steps between each injection.

In the classic approach, all the reagents are added by manual pipetting: a time-consuming process that greatly increases the likelihood of errors occurings. Using microfluidic systems overcomes the limitations of conventional protocols. Automating the sequential steps by using our pressure-based flow controllers coupled with rapid low-volume valves not only saves time for the biologist, but also greatly improves the reproducibility of the assay. Switching from a bulk assay to a microfluidic format also allows for reduced reagent and sample volumes and shorter incubation times.

Our Immunostaining Pack combines all the equipment, software, and information you need to easily transfer your experiment from a bulk protocol to a microfluidic assay.

Features of the sequential injection pack

Complete system

All the components you need for your experiment are included in the Immunostaining Pack.

Versatility

This package can be used for diverse applications, and with any microfluidic chip.

The reservoirs can be easily manipulated for heating, agitation, etc.

Stable flow rate & short switching time

Fluigent’s products offer very stable delivery and short switching times to ensure optimal results for any immunostaining experiment.

Large sample volumes

Ten injection reservoirs allow for easy manipulation of large sample volumes, and our pressure-based flow controller offers a wide range of pressure and flow rates.

Dedicated protocol for sequential injections

Up to 10 selected liquids can be delivered sequentially to a chip using the M-SWITCH. A 2-SWITCH can also be used to sort the samples at the outlet of the chip to a collection tube or to waste.

Build customized and time-based protocols to save time and reduce variability with our user-friendly OxyGEN software, designed for easy and complete protocol automation.

Related applications

Immunostaining

Chip preparation : Chip coating, cell loading, cell incubation

Main components of the Immunostaining Pack

Figure 1: Complete setup for an immunostaining experiment.

Pack Specifications

PRODUCTS

Our Immunostaining Pack includes the following components:

LineUp Flow EZ pressure controller (1000 mbar) (x1)

LineUp LINK Module (software control) (x1)

FLOW UNIT M (x1)

2-SWITCH (x1)

SWITCH EZ (x1)

P-CAP series 15 mL (x12)

M-SWITCH (x1)

10 Position Pressure Manifold (x1)

Immunostaining Pack setup — *Fig 2 : Complete Schematic of the Immunostaining Package*

Setup Immunostaining Package with manifold — *Fig 3 : Complete Setup of the Immunostaining Package*

FLUID HANDLING SYSTEM

Product	Part number
1*Flow EZ	LU-FEZ-1000
1*2-SWITCH	2SW003
1*SWITCH EZ	ELUSEZ
1*LINK module	LU-LNK-0002
1*M-SWITCH	ESSMSW003

RESERVOIRS

Product	Part number
12*15 mL Pcap with 15 mL Falcon tube	P-CAP15-HP
1*10 Position Pressure Manifold	CTQ-MANI

FLOW METERS

Product	Part number
1*Flow unit M	FLU-M-D

TUBING

Product	Part number
Tubing and connection Kit P-CAP 15mL FEP tubing with an ID of 500 microns	CTQ-KIT-PC15
1*Tubing and connection Kit 2-SWITCH	CTQ-KIT-2SW2
1*Tubing and connection Kit Flow UNIT M	CTQ-KIT-LQ
1*Tubing and connection Kit M-SWITCH	CTQ-KIT-ESSMSW003

OxyGEN

Real-time control, automated protocol, data record and export

ver. 2.2.0.0 or more recent

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Software Development Kit

Custom software application

ver. 22.2.0.0 or more recent

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“We have been using Fluigent’s M-SWITCH, amongst other accessories, including Flow EZ system, flow controllers, for the past 5 years. We are pleased with its performance, especially the programmability and automation of fluid flow that relieves the user of the need to be present next to the experiments that run for several hours. We found the software interface to be simple to use and could count on the team’s assistance for friendly recommendations and support.”

Sivashankar Krishnamoorthy – Luxembourg Institute of Science and Technology (LIST)

See more about M-SWITCH

Aria: a fully integrated system

Run sequential perfusion and immunostaining experiments in an automated, intuitive, and completely integrated system with our ready-to-use Aria system.

The Aria is our automated solution for cellular perfusion or timed injection protocols, allowing for the automatic delivery of up to 10 different solutions into a chamber or microfluidic chip, according to user-defined protocols.