Omi, an Automated Organ-On-A-Chip Platform

[O-OMISC-PTF]

New release

Mimic Microphysiological Conditions in Organ-on-a-Chip Studies

The Omi is an automated platform that helps control the microphysiological fluidic environment of organ cells inside microfluidic chips. With multiple protocol functions (perfusion, recirculation, sampling, and drug delivery), the Omi ensures precise shear stress control and supports long-term recirculation. It is compatible with any type of chip to sustain different cell culture types or organ-on-a-chip models (liver, gut, skin, etc.).

Ask for a quote Specifications

Main benefits

Designed for biology
Suited for cell culture under sterile conditions.
Versatile
Can be used with virtually any microfluidic chip design.
Efficient
Automated and fully integrated platform (Shear stress, flow rate, and pressure control).
Easy-to-use
Intuitive interface and technology for beginners and advanced researchers.

Applications: Control Your Experiment With Fluigent’s Omi

The Omi is ideal for cellular organ models, drug discovery, ADME-Tox and Safety.

Single Channel Configuration

Blood Vessel Reproduction: Reproduce precise shear stress conditions of blood vessels with endothelial cells-on-chip using the Omi.

HUVEC cells before connexion to Omi — HUVEC cells before connection to Omi

HUVEC after 7 days of culture media recirculation with Omi

Microfluidic Application Notes
Endothelial Cell Culture Under Shear Stress Using Omi
Read more

Gut-on-chip: Achieve optimal flow control and sustained culture conditions, enhancing cell differentiation, nutrient delivery, and intestinal barrier function to accurately replicate the human intestine.

Caco2 cells before Omi — Caco2 cells before connection to Omi

Caco2 cells after 7 days of culture medium recirculation with Omi

Microfluidic Application Notes
Omi, automated organ-on-chip platform, the Gut-on-chip model development
Read more

Cancer Drug Discovery

Mimic the effect of drug compounds on tumor cells to evaluate their response to treatments.

Double Channel Configuration

Liquid / Liquid interface:

When the Omi’s fluidic system is paired in two, it allows users to model liquid-liquid interfaces to reproduce physiological functions of different human organ models.

Blood-Brain-Barrier:

Assess vascular permeability and brain cells-on-chip. Our microfluidic functionalities and protocols allow for accurate cell organization and physiology to study the microenvironment of the human brain. 

Discover more about organ-on-a-chip technology and its diverse applications with our white paper.

« I’m thrilled to share that I’ve used Omi on numerous occasions for my cell biology experiments as part of my PhD. Omi is the simplest tool I’ve been able to use for on-chip organ recirculation and perfusion. Everything is integrated into a single device, and the sterile consumables are easy to use, which greatly reduces the risk of contamination.
Tablet and web applications allow real-time monitoring of experiments in progress, making it the perfect user-friendly tool. I’m really excited to be able to develop new biological applications with Omi! »

Arthur Salles – PhD Student

CNRS LIED – Team « Microsystèmes Cellulaires » – Université Paris Cité

Several Protocol Functions Can Be Produced with a Single OMI Platform

Perfusion

Perfuse up to 3 mL of several types of liquids such as cell culture media for long periods of time in a controlled and reproducible way. The Omi can perfuse culture media at low flow rates, making it easier to maintain sensitive spheroid and organoid cultures within microfluidic chips.

Recirculation

The device can produce unidirectional recirculation of mediawhile maintaining user defined flow rates with high precision. Read our technical note on long-term recirculation for organ-on-a-chip applications.

Sampling

Sample up to 3 mL of soluble factors secreted in the culture medium for analysis or imaging.

Injection

Inject up to 3 mL of fresh medium during an experiment.

Flow rate and pressure rate monitoring over time for 50 µL/min

Designed to fit in incubators, and easy to transport to a microscope for imaging or cell analysis

Completely autonomous platform

The Omi features a two-hour battery backup supplyfor uninterrupted operation. In addition, Wi-Fi or Bluetooth connectivity ensures easy setup and monitoring of your protocols through its user-friendly application interface. It can easily be transported from an incubator to a microscope to perform live cell imaging while maintaining cell perfusion under battery power.

Reproducibility and experimental versatility

The Omi can be used in different modes:

Single mode : single channel perfusion for cell culture, such as blood vessel

Dual mode: double channel perfusion for co culture to perform liquid/liquid interface or blood-brain barrier by synchronizing two Omis with one organ-on-a-chip.

Compact and Transportable: One incubator, several Omis

This space saving platform allows multiple Omis inside a single incubator for a given experiment. Units can be easily transported to a microscope for real-time monitoring while maintaining perfusion.

Contamination-free

Inject cell or fresh medium, deliver test compounds, collect samples without disconnecting the microfluidic chip and disturbing the experiments.

Specifications

PERFORMANCE

Flow rate control	From 1 µL/min to 1 mL/min*
Maximum pressure	600 mbar at maximum
Fluid reservoir volume	3 mL
Perfusion / Injection / Sampling volume	From 1 mL to 3 mL
Min volume recirculated	2 mL

* The flow rate depends on the microfluidic chip and the adaptator.

HARDWARE SPECIFICATIONS

*Dimensions (LWH)*	190 x 120 x 60 (mm)
Weight	694 g
Operating temperature	15-40°C
Gas input pressure	Atmospheric pressure
Gas input composition	Dry 2 µm-filtered air, O2/CO2 mix or incubator air

ELECTRONICAL SPECIFICATIONS

Power supply voltage	24 V DC
Max energy consumption	12 W
Max current requirement	0.5 A
Battery autonomy	2 h

CARTRIDGE SPECIFICATIONS

Dimensions	80 x 60 x 36 (mm)
Weight	56 g
Materials	Medical grade polycarbonate and MVQ silicone*
Working fluids	Water based solutions, IPA, Ethanol
Cleaning	Isopropanol, 80%/20% v/v water ethanol solution, tergazyme 1%

*Check the material compatibility of solutions with the wetted materials available in the Omi’s Datasheet

TUBING SPECIFICATIONS

Material	FEP (provided) , but other materials possible depending on the connector
ID	250 μm (provided), but other can be used depending on the application
OD	1/16” (provided), but other can be used with a compatible 1⁄4-28 flat bottom connector

Omi SOFTWARE (tablet)

Control in real-time, protocol automation, data record and export

ver. 1.2.3 or more recent

*Provided with the Omi device

https://play.google.com/store/apps/details?id=com.fluigent.omi

Omi WEB:

Manage your team and monitor your running experiments

https://omi.fluigent.com/login

1. Can I clean the disposable elements to reuse them?

No. These disposables are for one time use. If the disposables are reused, Fluigent cannot guarantee accurate results.

2. Can I use ethanol or isopropanol in my protocols?

No, only during cleaning and sterilization steps.

3. How many experiments can I run with the disposables without replacement?

One experiment can be run from several hours up to multiple weeks.

4. How can I clean the Omi?

For the external cleaning of the device, gently wipe the surface with a tissue and ethanol or isopropanol.

For the cleaning of the fluidic part, we highly recommend following the specific cleaning protocol that can be found in the Omi App protocol editor. Manual cleaning can be performed using the Omi manual mode by recirculating your own detergent through the system.

5. How many Omi devices can I control with my tablet?

Users can monitor up to 12 Omi devices with the tablet. In the Omi app on the homepage, up to six devices are visible at once. When you have more than six devices, you must scroll left to the second page to see the other devices. When launching an experiment with the Omi, you will receive data every two or three seconds from the six devices present on the homepage. The devices that are not visible on the homepage are monitored every 30 seconds.

6. Can I use my own tablet to control the Omi?

Fluigent provides a tablet with the purchase of an Omi. Other tablets can be used, but we do not guarantee the same performance.

Omi, an Automated Organ-On-A-Chip Platform